Plasmid_Backbone

Part:BBa_J72204

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-09-17)

ampicillin resistant split R6K vector w/ O16 HAs and FRT-Kan-FRT

ampicillin resistant split R6K vector with O16 homology and kanamycin resistance flanked by FRT sites. Requires expression of pir to replicate.

Referred to internally as pBjk2648r

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3940
    Illegal XbaI site found at 45
    Illegal XbaI site found at 1114
    Illegal PstI site found at 16
    Illegal PstI site found at 1652
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3940
    Illegal PstI site found at 16
    Illegal PstI site found at 1652
    Illegal NotI site found at 1756
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 3940
    Illegal BglII site found at 3949
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3940
    Illegal XbaI site found at 45
    Illegal XbaI site found at 1114
    Illegal PstI site found at 16
    Illegal PstI site found at 1652
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3940
    Illegal XbaI site found at 45
    Illegal XbaI site found at 1114
    Illegal PstI site found at 16
    Illegal PstI site found at 1652
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2402


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Categories
Parameters
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